Journal: Translational Oncology

Article Title: Novel IL-15 dendritic cells have a potent immunomodulatory effect in immunotherapy of multiple myeloma

doi: 10.1016/j.tranon.2022.101413

Figure Lengend Snippet: Antibodies used in Flow cytometry analysis

Article Snippet: CD112-PE , R2.525 , Miltenyi Biotec (CA,USA).

Techniques: Flow Cytometry, Control, Staining

Journal: Translational Oncology

Article Title: Novel IL-15 dendritic cells have a potent immunomodulatory effect in immunotherapy of multiple myeloma

doi: 10.1016/j.tranon.2022.101413

Figure Lengend Snippet: IL-15 mDCs showed the enhanced cytotoxicity by generation of potent autologous cytotoxic T lymphocytes, CIK cells, and NK cells against primary autologous myeloma cells (paired). (A) We analyzed CD138 + primary myeloma cells isolated from bone marrow aspiration samples of newly diagnosed multiple myeloma patients to assess the expression levels of MHC class I, MICA, MICB, ULBP1, ULBP2/5/6, HLA-E, CD38 (n = 12), and CD112, CD155, ICAM-1 and PD-L1 (n = 5) using flow cytometry. ( B) We measured the expression of the degranulation marker CD107a in CTLs, CIK cells, and NK cells generated by mDCs against primary autologous myeloma cells. ( C) The killing capacity of activated lymphocytes by mDCs against primary autologous myeloma cells was investigated using an LDH-release cytotoxicity assay without or with anti-MHC class I mAb (n = 3). The data indicate that CLTs, CIK cells, and NK cells generated by the IL-15 mDCs (6 days) and IL-15 mDCs (8 days) groups exhibited stronger cytotoxicity than those of the conventional mDCs group. Data are representative from three independent experiments. *P < 0.05; ** P < 0.01; *** P < 0.001 (One-way ANOVA, multiple comparisons test: Tukey for B. Multiple t tests – one per row for C).

Article Snippet: CD112-PE , R2.525 , Miltenyi Biotec (CA,USA).

Techniques: Isolation, Expressing, Flow Cytometry, Marker, Generated, Cytotoxicity Assay

Journal: Cell Death & Disease

Article Title: Deciphering the tumor immune microenvironment of imatinib-resistance in advanced gastrointestinal stromal tumors at single-cell resolution

doi: 10.1038/s41419-024-06571-3

Figure Lengend Snippet: a Cell communication analysis on TIGIT-NECTIN2 and BTLA-TNFRSF14 pair between different cell types in imatinib resistant and sensitive patients respectively. Expression of NECTIN2 ( b ), BTLA ( c ) and TNFRSF14 ( d ) in each cell type in imatinib resistant and sensitive patients respectively. e IHC analysis of NECTIN2, BTLA and TNFRSF14 between imatinib resistant (upper) and sensitive (bottom) patients. f Schematic diagram of the unique tumor-immune microenvironment of imatinib-resistance in advanced GIST.

Article Snippet: Finally, two experienced pathologists independently evaluated staining results for Foxp3 (Servicebio, GB11093), BTLA (Boster, A03149), TNFRSF14 (Affinity, AB2838686) and NECTIN2 (Boster, A08081-2).

Techniques: Expressing

Journal: iScience

Article Title: Heterogeneity of perivascular astrocyte endfeet depending on vascular regions in the mouse brain

doi: 10.1016/j.isci.2023.108010

Figure Lengend Snippet: Localization of nectin-2 δ in the mouse hippocampus (A) Localization of nectin-2δ (N2δ) in stratum lacunosum-moleculare of the hippocampus. a, artery/arteriole; b, vein/venule; and c, capillary. Arrowheads, arteries/arterioles; arrows, veins/venules; ∗, capillaries. Scale bars, 20 μm. (B) The ratio of nectin-2δ-positive blood vessels in the hippocampus is shown as a bar graph. (C) Co-localization of nectin-2δ and GFAP at artery/arteriole in stratum lacunosum-moleculare of the hippocampus. Scale bar, 20 μm. Line scan of fluorescence intensity of the white line (a–b). AU, arbitrary units. These images are representative of three independent experiments. See also Figures S9 and .

Article Snippet: Rat Anti-Mouse Nectin 2 Monoclonal Antibody, Unconjugated, Clone 502-57 , MBL International , Cat# D083-3, RRID: AB_590848.

Techniques: Fluorescence

Journal: iScience

Article Title: Heterogeneity of perivascular astrocyte endfeet depending on vascular regions in the mouse brain

doi: 10.1016/j.isci.2023.108010

Figure Lengend Snippet: Localization of nectin-2α/δ in the purified PV-AEF (A and B) Immunofluorescence images of the purified PV-AEF immunostained with the indicated Abs. The anti-nectin-2 Ab, which recognizes nectin-2α/δ, was used. The purified PV-AEF were identified by AQP4-positive and DAPI-negative cell fragments and vesicles. The areas encircled by the cyan dotted line indicate the areas of the purified PV-AEF. Scale bars, 5 μm. (C and D) Mean fluorescence intensity of nectin-2 and AQP4 was shown in the bar graphs. The area of PV-AEF was measured by the AQP4 signal and divided into two groups according to the median value. Data are presented as mean and SD (n = 28, 28), and dot shows each data. The Mann-Whitney U test was performed. These images are representative of three independent experiments.

Article Snippet: Rat Anti-Mouse Nectin 2 Monoclonal Antibody, Unconjugated, Clone 502-57 , MBL International , Cat# D083-3, RRID: AB_590848.

Techniques: Purification, Immunofluorescence, Fluorescence, MANN-WHITNEY

Journal: iScience

Article Title: Heterogeneity of perivascular astrocyte endfeet depending on vascular regions in the mouse brain

doi: 10.1016/j.isci.2023.108010

Figure Lengend Snippet:

Article Snippet: Rat Anti-Mouse Nectin 2 Monoclonal Antibody, Unconjugated, Clone 502-57 , MBL International , Cat# D083-3, RRID: AB_590848.

Techniques: Produced, Recombinant, Affinity Purification, Protease Inhibitor, Western Blot, Fluorsave, Bicinchoninic Acid Protein Assay, Double Staining, TUNEL Assay, Mass Spectrometry, Plasmid Preparation, Software